which has ion
concentrations nearly equal to those of human blood plasma and is
buffered at pH 7.40 with 50 mM trishydroxymethylaminomethane and 45
mM hydrochloric acid at 36.5C.
Department of Material Chemistry,
Graduate School of Engineering, Kyoto University
Wash all the bottles and wares
with 1N-HCl solution, neutral detergent, and ion -exchanged and
distilled water, and then dry them.
Put 500 ml of ion-exchanged and
distilled water into one liter polyethylene bottle, and cover the
bottle with a watch glass.
Stir the water in the bottle
with a magnetic stirrer, and dissolve the reagents one by one in
the order as given in Table 1 (One after the former reagent was
completely dissolved).
Adjust the temperature of the
solution in the bottle at 36.5 with a water bath, and adjust pH
of the solution at pH 7.40 by stirring the solution and titrating
1N-HCl solution (When the pH electrode is removed from the
solution, add the water used for washing the electrode to the
solution).
Transfer the solution from the
polyethylene bottle to a volumetric glass flask. Add the water
used for washing the polyethylene bottle to thew solution in the
flask.
Adjust the total volume of the
solution to one liter by adding ion-exchanged and distilled water
and shaking the flask at 20.
Transfer the solution from the
flask to a polyethylene or polystyrene bottle, and store the
bottle in a refrigerator at 5-10 (If some substance is
precipitated in the solution during the storage, do not use this
solution as SBF and its container again).
Table 1. Ion concentrations (mM)
of SBF and juman blood plasma
Ion
Simulate Body
Fluid
Blood plasma
Na+
142.0
142.0
K+
5.0
5.0
Mg2+
1.5
1.5
Ca2+
2.5
2.5
Cl-
148.8
103.0
HCO3-
4.2
27.0
HPO42-
1.0
1.0
SO42-
0.5
0.5
Table2. Regents for preparing SBF (pH7.40,
1L)
Order
Reagent
Amount
1
NaCl
7.996 g
2
NaHCO3
0.350 g
3
KCl
0.224 g
4
K2HPO43H2O
0.228 g
5
MgCl26H2O
0.305 g
6
1M-HCl
40 mL
(About 90 % of total amount
of HCl to be added)
7
CaCl2
0.278 g
8
Na2SO4
0.071 g
9
(CH2OH)3CNH2
6.057 g
@
Figure 1. Preparation of SBF.
@
T. Kokubo, H. Kushitani, S. Sakka, T.
Kitsugi and T. Yamamuro, "Solutions able to reproduce in vivo
surface-structure changes in bioactive glass-ceramic A-W",
J. Biomed. Mater.
Res., 24, 721-734 (1990).