Bio-instrument Analysis

The course will begin with basic skills in handling organic compounds, before moving on to the isolation, refinement and identification of biological material. Students will then learn the use of various instruments used for structural analysis, which they will operate using actual compounds. Data processing will be performed on computers connected to the analytical instruments. Students will also learn about software for molecular model simulations.

・Handling of low molecular organic compounds
・Analysis of low molecular organic compounds using infrared and ultraviolet absorption spectrum instruments
・Analysis of the molecular structure of organic compound using nuclear magnetic resonance spectrum instruments
・Determining the molecular mass of protein using mass spectrometry
・Isolation of compounds using gas chromatography and high-performance liquid chromatography
・Analysis of amino acid sequences of proteins by separating protein sequencers
・Analysis of gene sequence using DNA sequencers

Lectures will be given using books and reference material specified in the package. Progress will be presented in seminar style followed by written submission as a report. Experiments will take place according to the procedure specified in the package. Reports will be made once per week and meetings will be called as needed.

Exercise problems related to the theme will be decided upon through private consultation with each student

This course will first focus on acquiring basic skills for the handling of low molecular organic compounds, proteins and genes. Students will then deepen their understanding of the principles for the most advanced analytical instruments that are actually used for research in the field of life science and biotechnology, and also about information processing methods using computers.

It is desirable that students have a basic knowledge of organic chemistry or protein chemistry, and molecular biology.

The following list shows examples of research projects that can be combined with this course.
"Isolation, analysis and identification of various organic compounds"
"Analysis of the structure and function of target proteins"
"Analysis of the structure and function of enzymes"
"Analysis of the base sequence of target genes"

Scores will be given on a scale of 1 to 5 for the items listed below. An average score of 3.5 is considered a passing score.

1. (1) Level of understanding of the basics of spectroscopy
   
2. (2) Level of understanding of skills for the use of instruments for analytical spectroscopy
   
3. (3) Level of understanding of chromatography
   
4. (4) Final presentation and report